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Bioconjugate Techniques
Greg T. Hermanson (Author)
Bioconjugate Techniques is an essential guide to modifying and crosslinking biomolecules for use in research, diagnostics, and therapy. It provides highly detailed information on chemistries, reagent systems, and practical applications for creating labeled or conjugated molecules. It also describes dozens of reactions, with details on hundreds of commercially available reagents, and the use of these reagents to modify or crosslink peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers. Armed with this information and the extensive protocols provided, the reader will form their own conjugates that can be used to detect, quantify, and target important analytes.
This book will help readers make: biotinylated molecules, avidin or streptavidin conjugates, colloidal gold-labeled proteins, PEG or dextran conjugates, labeled oligonucleotide probes, and fluorescently or radioactively labeled molecules.
Publisher: Academic Press; 1st edition